• 专利附录
  • 专利说明
  • 权利要求
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    • 专利摘要:
    Syn-isomers of 7-substituted-3-vinyl-3-cephem compounds of the formula <CHEM> in which R<1> is amino or a protected amino group, and R<2> is carboxy or a protected carboxy group, and a pharmaceutically acceptable salt thereof, and processes for their preparation and pharmaceutical compositions, containing them as an active ingredient in admixture with pharmaceutically acceptable carriers.
    公开号:SU1309911A3
    申请号:SU833649764
    申请日:1983-09-29
    公开日:1987-05-07
    发明作者:Такаяа Такао；Такасидзи Хисаси；Масудзи Такаси；Яманака Хидеаки；Кавабата Кохи
    申请人:Фудзисава Фармасьютикал Ко.,Лтд (Фирма)；
    IPC主号:
    专利说明:

    The invention relates to a method for the preparation of new antibiotics of the cephalosporin series, namely, 7-substituted 3-vinyl cephalosporins or their acid addition salts, which can be used as antimicrobial agents in medicine.
    The purpose of the invention is to obtain new cephalosporin antibiotics, which have improved properties. . ,
    Example 1. To a solution of 48 g of benzhydryl complex 7- (4-bromo-2-oxyiminoacetamido) -3-vinyl-3 cephem-4-carboxylic acid in 200 MP N, N-dimethylacetamide was added 7.0 g of thiourea at 5 ° C and the mixture is stirred at room temperature for 1 hour. The reaction mixture is then poured into a 3% aqueous solution of sodium bicarbonate (2 L) and 150 g of sodium chloride is added to the mixture. The precipitate is collected by filtration and then dissolved in a mixture of 200 ml of acetone and 500 MP of ethyl acetate. The separated organic layer is washed with an aqueous solution of sodium chloride and then evaporated. The resulting residue is collected by filtration, washed with ethyl acetate and diethyl ether and dried under vacuum. obtaining benzhydryl ester (2-aminothiazole-4-Sh1) -2-oxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid, syn isomer (16.9 g), m.p. 133-136 S.
    IR spectrum (nudzhol), cm: 3200; 780i 1720, 1670; 1610.
    I R spectrum (DMSO-dt) 3.75 (2H, m) -, 5.2-6.1 (4H, m) ;. 6.67 (GN, s); 6.75 (1H, m); 7.00 (1H s); 7.20 (2H, m); 7.34 (UN, m); 9.50 (1H, d, Hz).
    Example 2: The following compounds are prepared analogously to Example 1,
    1) Bb Ethoxycarbonyloxyethyl ester (2-aminothiazol-4-yl) -2-hydroxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer).
    IR spectrum (nudzhol), cm: 3300; 1780; 1750; 1670.
    NMR spectrum (DMCO-d j), cG J 1.17 (3N, t, Hz); 1.50 (3N, d, Hz); 3.75 (2H, m); 4.13 (2H, k, Hz); 5.1-6.0 (4H, m); 6.63 (1H, s); 6.7-7.3 (4H, m); 9.45 (1H, d, Hz); 11.33 (1H, s).
    2) tert-Butoxycarbonylmethyl ester (2-aminothiazol-4-yl) -2-ok
    Siiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer). IR spectrum (nudzhol), cm 3300; 3170; 1780; 1730; 1665; 1620.
    3) DL-1-Propionyloxyethyl ester of 7-C2- (2-aminothiazol-4-yl) -2-hydroxyiminoacetamido3-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer).
    IR Spectrum (Nujol), 3300; 3200; 1780; 1765; 1720; 1710; 1660; 1630.
    4) 7- (2-aminothiazol-4-yl) -2-hydroxyimino-acetamido-3-vinyl-3-cephem-4-car-5-sidelic acid pivaloyloxymethyl ester (syn-isomer).
    IR spectrum (nudzhol), cm: 3400; 1785; 1750; 1670; 1615; 1530; 1310; 1220.
    5) 7-C2- (2-aminothiazol-4-yl) -2-hydroxyiminoacetamide-3-vinyl-3-cephem-4-carboxylic acid palmitoyloxymethyl ester (syn-isomer).
    Zh-spectrum (nudzhol), cm: 3300; 1775; 1670; 1615; 1530; 1305; 1210.
    6) (5-Methyl-2-oxo-1, 3-dioxol-4-yl) methyl ester 7-C2- (2-aminothiazol-4-yl) -2-oxyiminoacetamido - -3 Vinyl-3- cef-4-carboxylic acid (syn-isomer).
    IR spectrum (nudzhol), cm: 3300; 1812; 1772; 1730; 1668; 1611.
    7) (2-Aminothiazol-4-yl) -2-oxyiminoacetate-amino-3-vinyl-3-cephem-4-carboxylic acid phthalide-3-yl ester
    5 acid (syn-isomer).
    IR spectrum (nudzhol), cm: 3200 (width peak); 1772 (shooting range. Peak); 1728 (shoulder); 1660, 1620.
    8) 0 (2-aminothiazol-4-yl) -2-oxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid carboxymethyl ester (syn-isomer).
    IR spectrum (nudzhol), 1765 (broad); 1720, 1660 (broad).
    45 9) Sodium salt of 7-2-t2-aMHHO-thiazol-4-yl) -2-oxyiminoacetamido - -3-vinyl-3-cephem-4-carboxylic acid (syn-isomer).
    IR Spectrum (Nujol), 3200;
    50 1760; 1660; 1600
    EXAMPLE 3 (2-Aminothiazol-4-yl) -2-hydroxyiminoacetamido-3-vinyl-3-cephem-55-4-carboxylic acid benzhydryl ester was added parts to a mixture of 60 ml of 2,2,2-trifluoroacetic acid and 60 ml of anisole at. The mixture is then stirred at 5 ° for
    0
    five
    313
    1 h. The reaction mixture is added dropwise to 1.5 L of diisopropyl ether. The precipitate is collected by filtration. Then it is dissolved in a mixture of 100 ml of tetrahydrofuran and 100 ml of ethyl acetate and the solution is extracted with an aqueous solution of sodium bicarbonate. The resulting aqueous layer is acidified to pH 5.0 with 10% hydrochloric acid, washed with ethyl acetate and then chromatographed on alumina. The elution is carried out with a 3% aqueous solution of sodium acetate and the fractions containing the desired compound are collected. After acidification to pH 6.0 with 10% hydrochloric acid, the aqueous solution is again chromatographed on activated bone char. Zelation is carried out with 20% aqueous acetone. Selected fractions are concentrated in vacuo and then lyophilized to obtain the sodium salt of (2-aminothiazol-4-yl) -2-oxyiminoacetamido J-3-vinyl-3-cephem-4-carboxylic acid (syn - isomer, 14.4 g), which decomposes starting from 220 C.
    IR Spectrum (Nujol), 3200; 1760; 1660; 1600
    NMR (),: 3.67 (2H, s) 5.2-5.7 (3N, m); 5.83 (1K, d, T 5 Hz); 6.8 (W, m), 7.0 (1H, s).
    EXAMPLE 4 I-DL-IODZTIL ethyl ester carbonate ester (22 g) was added dropwise to a solution of 15 g of sodium salt (2-aminothiazol-4-yl) -2-oxyiminoacetamido-3-vinyl- 3-cephem-4-carboxylic acid (syn-isomer) in N, N-dimethylformamide (120 ml at 5-7 ° C). The mixture is stirred at 30 minutes. 200 ml of ethyl acetate is added to the reaction mixture and then filtered. The filtrate is washed with water and an aqueous solution of sodium chloride and dried over magnesium sulphate. After removing the solvent, the residue is washed with ethyl acetate and dried in vacuo to give (2-aminothiazol-4-yl) -2-oxyiminoacetamido 3 -C-vinyl-3-cephem-4-carboxylic acid DL-1-ethoxycarbonyloxyethyl ester
    you, syn-isomer, 7.4 g, so pl. 126-130 ° C.
    IR Spectrum (Nujol), 3300; 1780; 1750; 1670; 1620.
    NMR spectrum (DMSO-d), (/: 1.17 (3N, t, Hz); 1.50 (3N, d, 1–6 Hz); 3.75 (2H, m); 4.13 (2H , k, Hz); 5.1-6.0 (4H, m); 6.65 (1H,

    about
    Q
    five
    five
    0
    five
    with); 6-7.3 (4H, m); 9.45 (1H, d, 1 8 Hz); 1.33 (IH, s).
    Example 5. Cesium carbonate (2.06 g) is added to a solution of 5 g of (2-aminothiazol-4-yl) -2-hydroxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer ) in 50 ml of M, 1 1-dimethylacetamide at 25 C.
    The mixture was stirred at room temperature for 1 hour and cooled in an ice bath. Ethyl carbonate 1-DL-iodoethyl ester (9.2 g) was added to this cooled mixture immediately, in one portion. The mixture is stirred for 40 minutes at. 300 ml of ethyl acetate is added to the reaction mixture, after which it is filtered. The filtrate is washed twice with water and brine, treated with activated bone char and dried over magnesium sulfate. After removal of the solvent in vacuo, the residue is washed with diisopropyl ether and air dried to afford 7- -2- (2-aminothiazol-4-yl) -2-oxy-1-chloroacetamido-3-vinyl-3-cephem DL-1-ethoxycarbonyloxyethyl ether -4-carboxylic acid, syn-isomer, 4.6 g, m.p. 126-130 s.
    IR spectrum (nudzhol), cm: 3300, 1780-, 1750; 1670.
    NMR spectrum (DMCO-dt), cG; 1.17 (3N, t, Hz); 1.50 (3N, d, Hz); 3.75 (2R, m); 4.13 (2H, k, Hz); 5.1-6.0 (4H, m); 6.63 (1H, s); 6.7-7.3 (4H, m); 9.45 (1H, d, Hz);
    11.33 (1H, s).
    I
    Example 6 Potassium iodide (4.0 g) was added to a solution of 1.2 g of tert-butyl chloroacetate in 50 ml of N, N-dimethylacetamide; The mixture is stirred for 40 minutes at room temperature. The precipitate is filtered off. The potassium salt of (2-aminothiazol-4-yl) -2-hydroxyimino acetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer, 3.2 g) is added to the filtrate at room temperature and the mixture stirred for 1.5 hours at the same temperature. The reaction mixture is added to a mixture of water and ethyl acetate and adjusted to pH 7.0 with a 20% aqueous solution of potassium carbonate. The organic layer was washed with water, dried over magnesium sulfate, and evaporated to give the t-butoxycarbonyl methyl ester, (2-aminothiazol-4-yl) -2-oxyimino
    513
    Acetamido3-3-vinyl-3-cephem-4-carboxylic acid, syn-isomer, 2.0 g
    IR Spectrum (nudzhol), 3300, 3170; 1780; 1730; 1665; 1620.
    NMR spectrum (DMCO-dfc), cG: 1.43
    (9H, s); 3.76 (2H, k, Hz); 4.73 (2H, s); 5.24 (1H, d, 0 Hz); 5.38 (W, d, Hz); 5.68 (1H, d, Hz); 5.82 (1H, d, and 8 Hz); 6.66 (H, s); 7.03 (1H, dd, and 18 Hz); 9.46 (N, d, 0 Hz).
    PRI me R 7. DL-1-Propionyloxyethyl ester (2-aminothiazol-4-yl) -2-hydroxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid (syn isomer, 1 , 38 g) is obtained by the interaction of (2-aminothiazol-4-yl) -2-oxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer, 5 g) with DL-1-bromoethylpropionate
    (4.56 g) in the same way as in tsimer 5.
    IR spectrum (nudzhol), cm 3300; 3200; 1780; 1765; 1720; 1710; 1660; 1630.
    NMR spectrum (DMCO-d), cG: 1.03 (3N t, Hz); 1.48 (3N, d, Hz);
    2.38 (2H, k, Hz); 3.53 and 3.97 (2H, ABc, Hz); 5.23 (1H, d, Hz); 5.4 (1H, d, Hz); 5.65 (1H, d, Hz); 5.85 (1H, dz, and 5 Hz); 6.67 (1H, s); .6.83 (1H, dd, and P Hz); 6.93 (1H, k, Hz); 7.1 (2H, br, s); 9.43 (1H, d, 1 8 Hz); 11.33 (W, s).
    Example8. 1.24 g of (2-aminothiazole-4-yl) -2-oxyiminoacetamido3-3-vinyl-3-cephem-4-carboxylic acid pivaloylsimethyl ester (7-synomer) is obtained by reaction of 7- C2- (2-aminothiazol-4-yl) -2-hydroxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer, 3 g) with iodomethyl pivalate (5.05 g) according to the method of example 5, so pl. 90-lOO c (decomposition).
    IR Spectrum (nudzhol), 3400;
    1785; 1750; 1670; 1615; 1530; 1310; 1220.
    NMR spectrum (QMCO-d, 1.14 (9H s); 3.58 and 3.97 (2H, AB k, Hz); 5.24 (1H, d, Hz); 5.39 (1H, d , Hz; 5.7–6.0 (ZN, m); 5.77 (1H, d, Hz); 6.70 (1H, s); 6.83 (1H, dd, Hz, and 17 Hz ); 7.1 (2H, width
    with); 9.49 (1H, d, Hz); 16.24
    pH, c).
    PRI me R 9. 1.86 g of palmitoyl-hydroxymethyl ester (2-aminothiazol-4-yl) -2-oxyiminoacetamido1-3
    50
    five
    about
    Q


    five
    five
    sixteen
    -Vinyl-3-cephem-4-carboxylic acid (syn-isomer) is obtained by reacting 3 g of (2-aminothiazol-4-yl) -2-oxyiminoacetamide J-3-vinyl-3-cephem-4-carboxylic acid ( syn-isomer) with 4,13 iodomethyl palmitate according to the method of example 5, so pl. 90-105 ° C (with decomposition).
    IR spectrum (nudzhol), cm: 3300; 1775; 1670; 1615; 1530; 1305; 1210.
    NMR spectrum (DMSO-d), /: 1.1-1.7 (26H, m); 2.3-2.5 (2H, m); 3.56 and 3.95 (2H, AB c, Hz); 5.21 (1H, d, Hz); 5.37 (1H, d, Hz); 5.7-6.0 (ЗН, m); 5.75 (W, d, 1 17 Hz); 6.66 (H, s); 6.7-7.0 (1H, m).
    I
    Example 10. To a solution of 2.0 g
    the potassium salt of 7-C2- (2-aminothiazol-4-yl) -2-oxyimine ope m amido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer) in 30 MP K, K- dimethylacetamide was added 1.0 g of 4-bromomethyl-5-methyl-1,3-dioxol-2-one (1.0 g) with ice-cooling and stirring. The reaction mixture was stirred at the same temperature for 30 minutes. The resulting mixture is taken up in 200 MP of ethyl acetate and the organic solution is washed three times with water. The organic layer is dried over magnesium sulphate and concentrated under reduced pressure. The residue is chromatographed on 50 g of silica gel to give 0.62 g of (5-methy-2-oxo-1,3-dioxol-4-yl) methyl ester of 7-C 2- (2-aminothiazole-4-yl) - 2-oxyiminoacetamido 3-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer).
    NC spectrum (nudzhol), 3300; 1812, 1772-, 1730; 1668; 1611.
    NMR spectrum (DMSO-dt), s /: 2.17 (3N, s); 3.52 and 3.98 (2H, AB k, 1 17 Hz); 5.15 (2H, s); 5.2o (1H, d, Hz); 5.3 (1H, d, Hz) j 5.63 (1H, d, Hz); 5.76 (1H, dd, 1 5, and 8 Hz); 6.63 (W, s); 6.83 (H, dd, and 17 Hz); 9.42 (W, d, 1 8 Hz); 11.3 (1H, s).
    Example P. 1.05 g of (2-aminothiazol-4-yl) -2-oxyiminoacetamido 3-vinyl-3-cephem-4-carboxylic acid phthalide-3-yl ether (3-vinyl-3-cephamic acid) (syn-isomer) is obtained by reaction potassium salt - (2-aminothiazol-4-yl) -2-oxyimino-acetamido-3-linyl-3-cephem-4-carboxylic acid (syn-isomer, 1.0 g) c
    0.9 g of 3-bromophthalide according to the method of example 10.
    Zh-spectrum (nudzhol). 3200 (width 1772 (width); 1728 (shoulder); 1660.1620
    NMR spectrum (VMSO-a,), cL: 3.7 (2H, m); 5.18 (1H, d, Hz); 5.43 (1H, d, Hz); 5.73 (1.H, d, Hz); 5.83 (1H, dd, and 8 Hz); 6.75 (1H, s); 6.7-7.2 (2H, m); 7.66-8.0 (6H, m); - 9.87 (1H, d, Hz).
    Example 12. 5.4MP of trifluoroacetic acid is added to a suspension of 1.8 g of tert-butoxycarbonylmethyl ester (2-aminothiazol-4-yl) -2-oxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid ( syn-isomer) in 4 ml of methylene chloride at room temperature. The mixture is stirred for 2 hours at the same temperature.
    Diisopropyl ether was added to the resulting solution and the mixture was stirred. The precipitate formed is collected by filtration and washed with diisopropyl ether. The precipitate is added to a mixture of ethyl acetate and water, and the mixture is neutralized to pH 7.0 with 20% aqueous sodium carbonate solution with stirring. The separated aqueous layer is acidified to pH -2.2 with 10% hydrochloric acid while cooling with ice. The precipitate is collected by filtration, washed with ice water and dried over phosphorus pentoxide in vacuum to obtain 0.73 g of carboxymethyl ether (2-aminothiazole). 4-yl) -2-oximino acetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer).
    IR spectrum (nudzhol), cm: 1765 (broad); 1720; 1660 (width).
    NMR spectrum (DMSO-d (,), 3.76 (2H c, Hz); 4.76 (2H, s); 5.24 (1H, d, Hz); 5.37 (1H, d, Hz ); 5.86 (W, d, Hz), 7.83 (1H, dd, and 8 Hz); 6.69 (W, s); 6.61-7.67 (GD, m); 9, 50 (1H, d, 0 Hz).
    Example 13. To a solution of I g of DL-1-ethoxycarbonyloxyethylBooth ester (2-aminothiazol-4-yl) -2-hydroxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer) in a mixture of 50 MP of ethyl acetate and 2 m of ethanol are added with 0.3 ml of concentrated hydrochloric acid under ice cooling. The mixture is stirred for 10 minutes at 0-3 C. To this solution is added 50 ml of diisopropyl ether, the precipitate formed is collected by filtration, washed with ethyl acetate and
    ten
    20
    25

    stitched in air to give 0.8 g of DL-1-ethoxycarboxy-ethyl ethyl ester of 7-t2- (2-aminothiazol-4-yl) -2-oxyiminoacetamidoL-3-vinyl-3-cephem-4-carboxylic acid hydrochloride (syn - isomer).
    IR spectrum (nudzhol), cm: 3100; 1780; 1750; 1640.
    NMR spectrum (DMCO-d g), 1, 23 (3N, t, Hz); 1.53 (3N, d, Hz); 3.75 (2H, m); 4.20 (2H, k, Hz); 5.0-6.0 (6H, m); 6.83 (H, s); 6.7-7.2 (2H, m); 9.7 (1H, d, Hz); 12.5 (W, br. S).
    Example G4. To a solution of 10 g of 7-4-bromoacetoacetamido-3-vinyl-3-cephem-4-carboxylic acid benzhydryl ester in a mixture of 70 ml of methylene chloride and 25 mp of acetic acid is added dropwise. 3.5 ml of isoamyl nitrite at a temperature of from -3 to -5 ° C. The mixture is stirred for 40 seconds at -5 s, then 4 g of acetyl-acetone is added and stirred for
     Of
    thirty
    40
    30 min at 5 ° C. To the reaction mixture are added 3 g of thiourea and, after stirring for 3 hours, 70 ml of ethyl acetate and 100 ml of diisopropyl ether are added dropwise. The precipitate was collected by filtration and dried in vacuo to give 11.7 g of benzhydryl ester - (2-aminothiazol-4-yl) -2-oxyimino-acetamido-3-vinyl-3-cephem-4-carboxylic acid, hydrobromide ( syn isomer). 3 g of this product is added in portions to a mixture of 5 ml of 2,2,2-trifluoro-acetic acid and 5 ml of anisole, at. After stirring for 1 hour while the reaction mixture is added dropwise to 150 ml of diisopropyl ether. The resulting precipitate is collected by filtration and dissolved in a mixture of 10 ml of tetrahydrofuran and 10 ml of ethyl acetate. The organic layer is extracted with an aqueous solution of sodium bicarbonate. The aqueous extract is washed with ethyl acetate, maintaining the pH at 5.0 and then acidified to. pH 2.2 with 10% hydrochloric acid. This solution is stirred for 1 hour at, the resulting crystals are collected by filtration and dried in vacuo to give (2-amy 55 notiazol-4-yl) -2-oxyiminoacetamido J-3-vinyl-3-cephem-4-carboxylic acid, syn- isomer, 0.79 g
    IR Spectrum (Nujol), 3300; 1780; 1665; 1180; 1130.
    45
    50
    Example 15. To a solution of 15 g of 7- (4-bromoaceto-acetamido) -3-vinyl-3-cephem-4-carboxylic acid benzhydryl ester in a mixture of 100 ml of methylene chloride and 30 ml of acetic acid are added dropwise A solution of 2.8 g of sodium nitrite in 5 ml of water at a temperature of -10 to. The reaction mixture is stirred for 40 minutes at -5 ° C, then A g of acetyl-acetone is added and then further stirred for 15 minutes at room temperature. The reaction mixture is added to a mixture of 200 ml of water and 200 m
    -
    methylene chloride, the organic layer is separated, and the npoivibiiset is washed with water. This solution is evaporated and the residue is dissolved in 40 ml of H, K-dimethylacetaceta. To this solution was added 3.4 g of thiourea, the mixture was stirred for 1 h at room temperature and poured into a mixture of 150 ml of tetrahydrofuran, 300 ml of ethyl acetate and 300 ml of water. This mixture is alkalinized to pH 6.0 with a 20% aqueous solution of sodium hydroxide.
    The separated organic layer is successively washed with a 20% aqueous solution of sodium chloride and dried over magnesium sulfate. The solvent is removed by distillation in vacuo, the precipitate is collected by filtration and washed with ethyl acetate and diisopropyl ether. This precipitate is dried, in vacuo, and 8.5 g of benzhydryl ester of (2-α-aminothiazol-4-yl) -2-hydroxyimoacetate-1-1 to-3-vinyl-3-cephem-4-carboxylic acid are obtained (syn-isomer ),
    IR spectrum (nudzhol), cm: 3200; 1780; 1720; 1670; 1610.
    Example 16. To a solution of 5 g of benzhydryl ester (2-aminothiazol-4-yl) -2-oxyiminoacetamido - -3-vinyl-3-cephem-4-carboxylic acid (syn-isomer) in a mixture of 20 mp anisole and 5 ml of acetic acid are added dropwise with 5 ml of boron trifluoride etherate at. After stirring for 20 minutes at 10 ° C, the reaction mixture is extracted into a mixture of 100 m of tetrahydrofuran, 100 ml of ethyl acetate and 00 ml of water and then basified to pH 6.0 with a 20% aqueous solution of sodium hydroxide. The resulting aqueous layer was separated and washed with ethyl acetate, maintaining the pH at 6.0. The solution is subjected to alumina chromatography. Coat 5 l
    t5
    20 25
    30 35
    40
    45 50 p 55 309911 .10
    eluting with 3% aqueous sodium acetate solution, collected and acidified to pH with 10% hydrochloric acid. This solution is additionally chromatographed on Diaion HP-20 non-ionic adsorption resin (trademark, product of Mitsu-bisi Chemical Industries). The fractions eluted with 20% aqueous acetone were collected, concentrated in vacuo, and acidified to pH 2.0 with 10% hydrochloric acid. The precipitate was collected by filtration and dried in vacuo to give (2-aminothiazolyl-4-yl) -2-oxyiminoacetamido j-3-vinyl-3-cephem-4-carboxylic acid, syn-isomer, 1.23 g,
    IR spectrum (nudzhol), cm: 3300; 1780; 1665; 1180; 1 30.
     NMR spectrum (DMCO-dfc), s /: 3.76 (2H, AB k, Hz); 5.2-6.0 (4H, m); 6.73 (1H, s); 6.8-7.5 (ЗН, m); 9.5 (1H, d, Hz); 11.4 (1H, broad s).
    Example 17 1. Benzhydryl ester hydrochloride of 7-amino-3-vinyl-3-cephem 4-carboxylic acid (1 kg) and 1.46 kg of 1.3-bis (triphyl Icyl urea) are dissolved in 8 l tetrahydrofuran. The mixture is cooled to 20 ° C. To this solution, 4-bromoacetoacetic acid bromohydride, obtained from 224 ml of diketene and 147 ml of bromine in methylene chloride at -20 ° C, is added, and the mixture is stirred for 30 minutes at -15 ° C. The reaction mixture is added to a mixture of 12 l of ethyl acetate and 6 liters of water. The organic layer is separated, washed with an aqueous solution of sodium chloride and then embedded in a vacuum. The resulting precipitate is stirred in 10 L of diisopropyl ether for 1 hour, the resulting crystals are collected by filtration and dried under vacuum to give 1.27 kg of β-bromoacetoacetamido-3-vinyl-3-cephem-4-carboxylic acid benzhydryl ether, m.p. , 133 137 C (with decomposition).
    2. To a solution of 500 g of 7-4-bromoacetoacetamido-3-vinyl-3-cephem-4-carboxylic acid benzhydryl ester in a mixture of 4.5 liters of methylene chloride and 1.7 liters of acetic acid is added dropwise a solution of g of nitrite sodium in 450 ml of water at a temperature of from -1.5 to -22 ° C. The reaction mixture is stirred for 7 min at -15 ° C,
    117 g of ethyl acetate are then added and then stirred at room temperature for 5 minutes.
    The reaction mixture is washed with water (2 x 6 L) and an aqueous solution of sodium chloride (6 L). 82.2 g of thiourea, dissolved in N, N-dimethyl acetate (1 L), is added to the separated organic layer, and the mixture is stirred 1 h at 36 ° C. After removing methylene chloride in vacuo, the residual oil is poured into a mixture of 3.5 L of tetrahydroLurane, 7 L of ethyl acetate and 4 L of ice water. This mixture is alkalinized to pH 6.0 with 10% aqueous sodium hydroxide solution. The separated organic layer is washed with water (2 x 4 l) and an aqueous solution of sodium chloride. Solvent
    is removed by distillation in vacuo and an oily residue is left-washed.
    Shies crystals are stirred in a mixture of 1.6 L of ethyl acetate and 2.4 L of diisopropyl ether for 1 hour at 0 ° C. The resulting crystals are collected by filtration, and 394.5 g of 7-C2- (2-aminothiazole-4-yl) -2-oxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid benzhydrilate 394.5 g are obtained. ).
    IR spectrum (nudzhol), cm: 3200; 1780; 1720; 1670; 1610.
    Example 18. B, L-1-Acetoxy-ethyl (2-aminothiazol-4-yl) -2-hydroxyiminoacetamidoJ-3-vinyl-3-cephem-4-carboxylate (syn-isomer, 1.12 g) is obtained by the interaction of 7-C2- (2-amy noziazol-4-yl) -2-oxyiminoacetamido 3 -Z-vinyl-3-cephem-4-carboxylic acid (syn-isomer, 5 g) with P, L-1-bromo- ethyl acetate (3.42 g) in the presence of cesium carbonate (2.04 g) according to the method described in example 5.
    IR spectrum (nudzhol), cm 3300; 1780; 1760; 1670; 1210.
    Example 19. To a suspension of D, L- -1-acetoxyethyl (2-aminothiazol-4-yl) -2-hydroxyimino acetamido3-3-v-1-3-cephem-4-carboxylate (syn isomer, 0.6 g) in ethyl acetate (30 mp), 68 mg of hydrogen chloride in isopropyl alcohol (0.37 ml) are added while cooling on ice. The mixture was stirred as a suspension at ambient temperature for 30 minutes and then diethyl ether (100 ml) was added. After 30 min, the solid precipitate is collected by filtration, which gives the hydrochloric acid salt

    Slots D, L-1-acetoxystil-7-f2- (2-aminothiazol-A-yl) -2-hydroxyiminoacetate-amido-3-vinyl-3-cephem-4-carboxylate, syn-isomer, 0.54 year
    IR spectrum (nudzhol), cm: 1780; 1760; 1620; 1210; 1070.
    NMR spectrum (DMCO-dt), c /: 1.50 (3N, d, Hz); 2.10 (ЗН, s); 3.80 (, H, m); 5.2-6.0 (4H, m); 6.5-7.2 (2H, m); 6.87 (H, s); 9.70 (1H, d, Hz); 12.43 (1H, broad s.).
    Example 20. To a suspension of 4.26 g of (2-aminothiazol-4-yl) -2-oxyiminoacetamidoJ-3-vinyl-3-cepheme-4-capanoic acid. (Syn-isomer) in 25 ml of water is added 4.26 ml of hydrochloric acid while cooling on ice. - The mixture is stirred for 1 hour at the same temperature. The solution is decanted and subsequently
    the current diethyl ether, acetone, hexane, get the powder. The powder was collected by filtration to give 4.30 g of the hydrochloric acid salt (2-am-5 notiazol-4-yl) -2-oxyiminoacetamido - -3-vinyl-3-cephem-4-carboxylic acid (syn-isomer).
    IR Spectrum (Nujol), 3200; 1760-1780; 1720; 1660-1680; 1625.
    NMR spectrum (DMSO-d t), (: 3.70 (2H, AB k, and 26 Hz); 5.22 (1H, Hz); 5.75 (1H, dd, and 5 Hz); 5, 30 (1H, d, Hz); 5.59 (W, d, Hz); 6.85 (1H, s); 6.70-7.17 (2H, m), 9.67 (1H, d, Hz); 12.34 (1H wide. S).
    EXAMPLE 21 Methane sulfonate (2-aminothiazol-4-yl) -2-hydroxyiminoacetamido-3-vinyl-3-ce 40 fem-4-carboxylic acid (syn-isomer 0.56 g) is obtained at the interaction of 0.5 g of 7- (2-aminothiazol-4-yl) -2-oxyimino-acetamido-3-vinyl-3-cephem-4-carboxylic acid with 0.158 g of methanesulphate slots according to the method of example 20.
    IR Spectrum (Nujol), 1760-1780 1630-1670; 1590; 1520.
    NMR spectrum (DMSO-d), s /: 2.40 (3N, s); 3.72 (2H, AB, K, and
    50 26 Hz); 5.22 (1H, d, Hz); 5.30 (1H, d, Hz); 5.59 (W, d, J 17 Hz); 5.60-5.90 (W, m); 6.86 (1H s); 6.67-7.17 (2H, m); 9.67 (W, d, Hz), 12.2 (1H, pir. S).
    5J Example 22. To a solution of 22 g of benzhydride-7-4-chloro-2-oxyimino-acetamido-3-vinyl-3-defem-4-carboxylate in 100 ml of K, H-dimethylacetamide add 3.2 g of thiourea at 5 ° C
    1313
    the mixture is stirred at room temperature for 1 hour. Then the reaction mixture is poured into 1 liter of 3% aqueous sodium bicarbonate, 75 g of sodium chloride is added thereto. The precipitates are collected by filtration, then dissolved in a mixture of 100 ml of acetone and 250 ml of ethyl acetate. The separated organic layer is washed with an aqueous solution of sodium chloride, then evaporated, the precipitates obtained are collected by filtration, washed with ethyl acetate and diethyl ether and dried in vacuo. 7.5 g of benzhydride-7-f2 .- (2-am notiazol-4-yl) -2-hydroxyimoacetate-3-vinyl 3-cephem-4-carboxylate (syn isomer) are obtained. The chemical constants are identical to those of the compound of Example 1.
    In vitro antibacterial activity is determined by a double-folded agar plate by the dilution method, as described below.
    One loop of each test PTM overnight in trypticase soy broth (10 living cells in 1 ml) was cultured in a strip on heart stretching agar (HI agar) containing stepwise varying concentrations of the test compound. The minimum inhibitory concentration is determined after incubation for 20 hours at 37 ° C.
    Test compounds:
    (2-aminothiazol-4-yl) -2-hydroxyiminoacetamido-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer) - compound according to example 16;
    7- (2-phenyl-2-oxyiminoacetamido) -.-3-vinyl-3-cephem-4-carboxylic acid (syn-isomer) is a known compound.
    The test results are shown in the table.
    Test strain
    The minimum inhibitory effect is 1; and the concentration, g / ml.
    Staphylococcus
    aureus 209 P JC-1 0.2400
    Bacillus subtilis
    ATCC 6633 X3,13400
    14 Continuation of the table
    Test Subject
    Minimum inhibitory concentration, g / MP
    Escherichia coli
    JC-20,39400
    Proteus nirabilis 10,20400
    Proteus vulgaris 112,5. 400
    0
    five
    0
    five
    0
    five
    0
    five
    The acute toxicity LDjp of the compound of Example 6 (mouse 1CR, intravenously) is 1000 mg / kg.
    Urine excretion test. The test compound (100 mg / kg) is administered orally to groups of three rats and urine samples are taken within 0-24 hours.
    Test compounds:
    A.) 7- (2-aminothiazol-4-yl) -2-oxyimino-acetamido} -3-vinyl-3-cephem-4-carboxylic acid pivapoyloxymethyl ester (syn-isomer);
    B) 1-DL-ethoxycarbo nyloxyethyl ester (2-aminothiazol-4-yl) -2-oxioxy-1; etamido J-3-vinyl-3-tsfefem-4-carboxylic acid (syn-isomer).
    The test results — excretion values — with urine of compounds A and B,%:
    A54.09
    B26,0
    For therapeutic purposes, the target compound and its pharmaceutically acceptable salt are used in the form of a conventional pharmaceutical preparation that contains the compound as an active ingredient, in a mixture with pharmaceutically acceptable carriers, such as organic or inorganic, solid or liquid excipients, which are suitable for oral parenteral or external use. These pharmaceutical preparations can be in solid form, such as tablets, granules, powders, capsules, or in a liquid form, such as solutions, suspensions, solutions, emulsions, lemonade, and so on.
    If necessary, these drugs can be included
    151
    powerful substances, stabilizing agents, moisturizing agents and other commonly used additives, such as lactose, citric acid, tartaric acid, stearic acid, magnesium stearate, white earth, sucrose, corn starch, talc, gelatin, agar, pectic, oil ground nut, olive oil, cocoa butter, ethylene glycol, etc.
    The dosage of the proposed compound may vary, it depends on the age and condition of the patient, the type of disease, the type of compound that will be used, etc. The patient may be given daily dosages of between 1 mg and approximately 4000 mg or more. In the treatment of diseases caused by infection by pathogenic microorganisms, average individual doses of approximately 50, 100, 250, 500, 1000 and 2000 mg of the target compound can be used.


    I
    权利要求:
    Claims (1)
    [1]
    Invention Formula
    , / MS
    The method of obtaining 7-substituted 3-vinylcephalosporins of the general formula
    J-CONU-T-1 HiN S -V,
     ) sign
    in the form of syn-isomers,
    where R is hydrogen, or an alkali metal or an ester-forming group, is selected from benzhydryl, C —C-alkanoyloxy — C —C-apkyl, j3 —C-alkoxycarbonyl — C —C-alkyl, carboxy-hydroxy C-C-alkyl, C -Cj-alkoxycarbonyloxy-C-C-alkyl la, 3-phthalidyl or (5-methyl -2-OKCO-I, 3-dioxol-4-yl) methyl,
    or their acid addition salts,
    excluding and c. by that
    compound of general formula
    Editor V. Petrash Order 1809/58
    Compiled by Z. Lattova Tehred M. Khodanych
    Proofr G
    Circulation 372Subscribe
    VNIIPI USSR State Committee. . for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5
    Production and printing company, Uzhgorod, st. Project, 4
    sixteen
    ShgSO-S-SOSH-t-G
    P - - N
    about
    HE
    COOR
    o
    ,
    0
    in the form of syn-isomer,
    where R has the indicated meanings;
    X - halogen,
    are reacted with tirmo uve in an aprotic solvent medium, such as dimethylacetamide, at room temperature and, in the case of
     necessary, c. the target product, where R is the group forming the ester, or in its additive salt with an acid, remove the group forming the ester by hydrolysis to obtain the desired product, where R is hydrogen, or, if necessary, the target product, where R is hydrogen or alkali metal, transferred to the target product, where R is a group, the sample is complex
    5 The ether, by reaction with the corresponding halide compound, or, if necessary, by the desired product, where R is. -Appoxycarbonyl-C-C2-alkyl, is converted into the target
    a product, where R is carboxy-C;, is C2-alkyl, by hydrolysis and the desired product is isolated as the free acid, its alkali metal salt or ester as the free base or as an acid addition salt.
    Priority featured
    09/30/822 when R is hydrogen, alkali metal, benzhydryl, 1-propionic 1-OXYETS1 or C -Cj-alkoxycarbonyl-hydroxy-C -C-alkyl,
    26.08.83 when R - C - € 4-alkoxy-carbonyl-C-C / -alkyl, carboxy-C - C-alkyl, 3-phthalideshl, (5-methyl-2-ok-CO-1,3 - dioxole -4-yl) methyl or. - alkanonloxy-C-C-alkyl with the exception of 1-proxy-uloxyethyl.
    five
    0
    five
    Proofreader G. Reshetnik
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    同族专利:
    公开号 | 公开日
    DK427083D0|1983-09-19|
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    GB2127812B|1986-01-08|
    AU1927783A|1984-04-05|
    DK162718C|1992-05-11|
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    FR2533926B1|1986-05-02|
    GR79674B|1984-10-31|
    GB8325572D0|1983-10-26|
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    IE832273L|1984-03-30|
    IT1173673B|1987-06-24|
    EP0105459A2|1984-04-18|
    FR2533926A1|1984-04-06|
    JPS62294687A|1987-12-22|
    DE3379463D1|1989-04-27|
    IE56046B1|1991-03-27|
    SG61387G|1988-03-04|
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    JPH0657713B2|1994-08-03|
    AT381497B|1986-10-27|
    KR910003118B1|1991-05-18|
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    FI833370A|1984-03-31|
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    DK427083A|1984-03-31|
    KR840006254A|1984-11-22|
    GB2127812A|1984-04-18|
    DK162718B|1991-12-02|
    ES8800235A1|1987-10-16|
    FI74971B|1987-12-31|
    ES526091A0|1985-10-01|
    NO160080C|1989-03-08|
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    FR2461713B1|1979-07-19|1983-04-15|Roussel Uclaf|
    JPH051271B2|1982-01-22|1993-01-07|Fujisawa Pharmaceutical Co|
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    US4609730A|1982-11-22|1986-09-02|Fujisawa Pharmaceutical Co., Ltd.|7-[substituted imino-2--acetamido]-3-3-cephem-4-carboxylic acid , having antimicrobial activities|
    US4708955A|1985-06-24|1987-11-24|Bristol-Myers Company|3-propenyl-7-aminothiazol-ylcephalosporanic acids and esters thereof|
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    JP4544692B2|2000-04-13|2010-09-15|大塚化学株式会社|Method for producing 3-vinyl-cephem compound|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    US42897082A| true| 1982-09-30|1982-09-30|
    GB838323034A|GB8323034D0|1983-08-26|1983-08-26|7-substituted-3-vinyl-3-cephem compounds|
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